Biotechnology Applications Test Paper by TEACHING CARE online tuition and coaching classes

Biotechnology Applications Test Paper by TEACHING CARE online tuition and coaching classes. Answers are in Bold option.

1. Three critical research areas of

biotechnology are:

(i) Providing the best catalyst in the form of improved

organism usually a microbe or pure enzyme.

(ii) Creating optimal conditions through engineering for

a catalyst to act, and

(iii) Downstream processing technologies to purify the

protein/organic compound.

Choose the correct statement

1. i only
2. ii only
3. iii only
4. i, ii, iii

 

2. The three options that can be thought

for increasing food production

(i) agro-chemical based agriculture

(ii) organic agriculture; and

(iii) genetically engineered crop-based agriculture

Which of the above option causes soil pollution and decrease of soil fertility

1. i only
2. ii only
3. iii only
4. i, ii, iii

 

3. Which of the following options regarding green revolution is /are correct

 

1. The Green Revolution succeeded in tripling the food supply
2. The Green Revolution was not enough to feed the growing human population.

• The Increased yields during Green Revolution have partly been due to the use of improved crop varieties, but mainly due to the use of better management practices and use of agrochemicals (fertilisers and pesticides).

 

1. i only
2. ii only
3. iii only
4. i, ii, iii

 

 

4. Genetic modification has:

(i) made crops more tolerant to abiotic stresses (cold, drought, salt, heat).

(ii) reduced reliance on chemical pesticides (pest-resistant crops).

(iii) helped to reduce post harvest losses.

(iv) increased efficiency of mineral usage by plants (this prevents early exhaustion of fertility of

soil).

(v) enhanced nutritional value of food, e.g., Vitamin ‘A’ enriched rice.

 

Which of the above options  is /are correct

1. i, iv only
2. i,ii only
3. ii, iii only
4. i,ii, iii,iv

 

 

5.Choose the options given below that are not false

 

1. Bt toxin is produced by a bacterium called Bacillus thuringiensis .
2. Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide

resistance to insects without the need for insecticides;

1. Bt toxin is a bio-pesticide.

1. a  only
2. b only
3. a, b only
4. a, b, c only

 

6. Select the incorrect staement

 

1. Some strains of Bacillus thuringiensis produce proteins that

kill certain insects such as lepidopterans (tobacco budworm, armyworm),

coleopterans (beetles) and dipterans (flies, mosquitoes).

1. B. thuringiensis form protein crystals during a particular phase of their growth.
2. These B. thuringiensis  protein crystals contain a toxic insecticidal protein.
3. the Bt toxin protein exist as active protoxins

 

 

7. The plant ‘Brassica napus’ has been modified to

produce ______.

1.Vitamin A

2.hirudin

3.lysine rich seeds

4.insulin

8.The process of RNA interference has been

used in the development of plants resistant to

1.Insects

2.Nematodes

3.Fungi

4.Viruses

9.The transgenic animals are those which have

1.foreign DNA in some of their cells

2.foreign DNA in all of their cells

3.foreign RNA in all of their cells

4.Both (A) and (C)

10.Transgenic plants are developed by

1.Introducing foreign genes

2.Introducing gene mutations

3.Deleting certain chromosomes

parts

4.Stopping spindle formation

11. Cry genes or Bt genes are obtained from

1.Cotton pest

2.Tobacco plant

3.Bacillus thuringiensis

4.E – Coli

12.Genetic engineering has been successfully

used for producing

1.Transgenic models for studying

new treatments for certain cardiac diseases

2.Transgenic mice for testing safety

of polio vaccine before use in

humans

3.Animals like bulls for farm work as

they have super power

4.Transgenic Cow-Rosie which

produces high fat milk for making

ghee

 

13.Tobacco plants resistant to a nematode have

been developed by the introduction of DNA that

produced (in the host cells)

 

1.An antifeedant

2.A particular hormone

3.Both sense and anti-sense RNA

4.A toxic protein

 

14.Some of the characteristics of Bt cotton are

1.Medium yield, long fibre and

resistance to beetle pests

2.Long fibre and resistance to aphids

3.High yield and resistance to

bollworms

4..High yield and production of toxic

protein crystals which kill dipteran

pests

 

15. Stirred-tank bioreactors have been designed for

1.Purification of the product

2.Addition of preservatives to the

product

3.Availability of oxygen throughout

the process

4.Ensuring anaerobic conditions in

the culture vessel

 

16.The first clinical gene therapy was given for

treating?

1.Rheumatoid arthritis

2.Chicken pox

3.Diabetes mellitus

4.Adenosine deaminase deficiency

 

17.The enzyme employed for amplification of DNA

during PCR is commercially obtained from

1.Trichoderma reesei

2.Bacillus licheniformis

3.Streptococcus pyogenes

4.Thermus aquaticus

 

18.A regulatory body working under MoEF for the

release of transgenic crops is

1.NBPGR

2.GEAC

3.NSC

4.NIPGR

 

19. Cry1 endotoxins obtained from Bacillus

Thuringiensis are effective against

1.Flies

2.Mosquitoes

3.Boll worms

4.Nematodes

 

20.Cry II Ab and Cry I Ab produce toxins that

Control

1.Tobacco budworms and

nematodes respectively

2.Corn borer and cotton bollworms

respectively

3.Cotton bollworms and corn borer

respectively

4.Nematodes and tobacco budworms

respectively

 

21. Bacillus thuringiensis forms protein crystals

which contain insecticidal protein. This protein

1.Binds with epithelial cells of midgut

of the insect pest ultimately killing it

2.Does not kill the carrier bacterium

which is itself resistant to this toxin

3.Is activated by acid pH of the

foregut of the insect pest

4.Is coded by several genes including the gene cry

 

22. What is true about Bt toxin?

1.Bt protein exists as active toxin in

the Bacillus

2.The inactive protoxin gets

converted into active form in the

insect gut

3.The concerned Bacillus has

antitoxins

4.The activated toxin enters the

ovaries of the pest to sterilise it and

thus prevent its multiplication

 

23. Which one of the following is a correct

statement?

1.The anticoagulant hirudin is being

produced from transgenic Brassica

napus seeds

2.Somatic hybridization involves

fusion of two complete plant cells

carrying desired genes

3″Bt” in “Bt-cotton” indicates that it

is a genetically modified organism

produced through biotechnology

4.”Flavr Savr” variety of tomato has

enhanced the production of

ethylene which improves its taste

 

 

24. Bt cotton is not:
25. A GM plant
26. Insect resistant
27. A bacterial gene expressing system
28. Resistant to all pesticides

 

25. C-peptide of human insulin is:
26. A part of mature insulin molecule
27. Responsible for formation of disulphide bridges
28. Removed during maturation of pro-insulin to insulin
29. Responsible for its biological activity.

 

26. GEAC stands for:
27. Genome Engineering Action Committee
28. Ground Environment Action Committee
29. Genetic Engineering Approval Committee
30. Genetic and Environment Approval committee

 

27. Alpha-1 antitrypsin is:
28. An antacid
29. An enzyme
30. Used to treat arthritis
31. Used to treat emphysema
32. A probe which is a molecule used to locate specific sequences in a mixture

of DNA or RNA molecules could be:

1. A single stranded RNA
2. A single stranded DNA
3. Either RNA or DNA
4. Can be ss DNA but not ss RNA

 

29. Choose the correct option regarding Retrovirus:
30. An RNA virus that can synthesise DNA during infection
31. A DNA virus that can synthesise RNA during infection
32. A ssDNA virus
33. A dsRNA virus

 

30. The site of production of ADA in the body is:
31. Bone marrow
32. Lymphocytes
33. Blood plasma
34. Monocytes

 

31. A protoxin is:
32. A primitive toxin
33. A denatured toxin
34. Toxin produced by protozoa
35. Inactive toxin

 

32. Pathophysiology is the:
33. Study of physiology of pathogen
34. Study of normal physiology of host
35. Study of altered physiology of host
36. None of the above

 

33. The trigger for activation of toxin of Bacillus thuringiensis is:
34. Acidic pH of stomach
35. High temperature
36. Alkaline pH of gut
37. Mechanical action in the insect gut
38. Golden rice is:
39. A variety of rice grown along the yellow river in China
40. Long stored rice having yellow colour tint
41. A transgenic rice having gene for – carotene
42. Wild variety of rice with yellow coloured grains

 

35. In RNAi, genes are silenced using:
36. ss DNA
37. ds DNA
38. ds RNA
39. ss RNA

 

36. The first clinical gene therapy was done for the treatment of:
37. AIDS
38. Cancer
39. Cystic fibrosis
40. SCID (Servere Combined Immuno Deficiency resulting form

deficiency of ADA)

 

37. ADA is an enzyme which is deficient in a genetic disorder SCID. What is

the full form of ADA?

1. Adenosine deoxy aminase
2. Adenosine deaminase
3. Aspartate deaminase
4. Arginine deaminase

 

38. Silencing of a gene could be achieved through the use of:
39. short interfering RNA (RNAi)
40. antisense RNA
41. by both
42. none of the above
43. The transgenic plant flavr savr tomato carries an artificial gene for
    (A) Delay ripening process
    (B) Longer shell life
    (C) Added flavours
    (D) All of these
44. Biopatents are ______.
    (A) Right to use invention
    (B) Right to use biological entities
    (C) Right to use products
    (D) Right to use process
45. A set standards used to regulate own or community activity in relation to biological world is
    (A) Biopotency
    (B) Biopiracy
    (C) Biowar
    (D) Bioethics
46. Biopiracy means
    (A) Use of biopatents
    (B) Thefts of plants and animals
    (C) Stealing of bioresources
    (D) Exploitation of bioresources without authentic permission
47. Bioethcs is related to
    (A) Preventing biopiracy
    (B) Regulation of unethical activities like gene cloning in animals
    (C) Preventing theft of living materials
    (D) Moral guidance to the problems in biology