Biotechnology Applications Test Paper by TEACHING CARE online tuition and coaching classes
Biotechnology Applications Test Paper by TEACHING CARE online tuition and coaching classes. Answers are in Bold option.
- Three critical research areas of
biotechnology are:
(i) Providing the best catalyst in the form of improved
organism usually a microbe or pure enzyme.
(ii) Creating optimal conditions through engineering for
a catalyst to act, and
(iii) Downstream processing technologies to purify the
protein/organic compound.
Choose the correct statement
- i only
- ii only
- iii only
- i, ii, iii
- The three options that can be thought
for increasing food production
(i) agro-chemical based agriculture
(ii) organic agriculture; and
(iii) genetically engineered crop-based agriculture
Which of the above option causes soil pollution and decrease of soil fertility
- i only
- ii only
- iii only
- i, ii, iii
- Which of the following options regarding green revolution is /are correct
- The Green Revolution succeeded in tripling the food supply
- The Green Revolution was not enough to feed the growing human population.
- The Increased yields during Green Revolution have partly been due to the use of improved crop varieties, but mainly due to the use of better management practices and use of agrochemicals (fertilisers and pesticides).
- i only
- ii only
- iii only
- i, ii, iii
- Genetic modification has:
(i) made crops more tolerant to abiotic stresses (cold, drought, salt, heat).
(ii) reduced reliance on chemical pesticides (pest-resistant crops).
(iii) helped to reduce post harvest losses.
(iv) increased efficiency of mineral usage by plants (this prevents early exhaustion of fertility of
soil).
(v) enhanced nutritional value of food, e.g., Vitamin ‘A’ enriched rice.
Which of the above options is /are correct
- i, iv only
- i,ii only
- ii, iii only
- i,ii, iii,iv
5.Choose the options given below that are not false
- Bt toxin is produced by a bacterium called Bacillus thuringiensis .
- Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide
resistance to insects without the need for insecticides;
- Bt toxin is a bio-pesticide.
- a only
- b only
- a, b only
- a, b, c only
- Select the incorrect staement
- Some strains of Bacillus thuringiensis produce proteins that
kill certain insects such as lepidopterans (tobacco budworm, armyworm),
coleopterans (beetles) and dipterans (flies, mosquitoes).
- B. thuringiensis form protein crystals during a particular phase of their growth.
- These B. thuringiensis protein crystals contain a toxic insecticidal protein.
- the Bt toxin protein exist as active protoxins
- The plant ‘Brassica napus’ has been modified to
produce ______.
1.Vitamin A
2.hirudin
3.lysine rich seeds
4.insulin
8.The process of RNA interference has been
used in the development of plants resistant to
1.Insects
2.Nematodes
3.Fungi
4.Viruses
9.The transgenic animals are those which have
1.foreign DNA in some of their cells
2.foreign DNA in all of their cells
3.foreign RNA in all of their cells
4.Both (A) and (C)
10.Transgenic plants are developed by
1.Introducing foreign genes
2.Introducing gene mutations
3.Deleting certain chromosomes
parts
4.Stopping spindle formation
- Cry genes or Bt genes are obtained from
1.Cotton pest
2.Tobacco plant
3.Bacillus thuringiensis
4.E – Coli
12.Genetic engineering has been successfully
used for producing
1.Transgenic models for studying
new treatments for certain cardiac diseases
2.Transgenic mice for testing safety
of polio vaccine before use in
humans
3.Animals like bulls for farm work as
they have super power
4.Transgenic Cow-Rosie which
produces high fat milk for making
ghee
13.Tobacco plants resistant to a nematode have
been developed by the introduction of DNA that
produced (in the host cells)
1.An antifeedant
2.A particular hormone
3.Both sense and anti-sense RNA
4.A toxic protein
14.Some of the characteristics of Bt cotton are
1.Medium yield, long fibre and
resistance to beetle pests
2.Long fibre and resistance to aphids
3.High yield and resistance to
bollworms
4..High yield and production of toxic
protein crystals which kill dipteran
pests
- Stirred-tank bioreactors have been designed for
1.Purification of the product
2.Addition of preservatives to the
product
3.Availability of oxygen throughout
the process
4.Ensuring anaerobic conditions in
the culture vessel
16.The first clinical gene therapy was given for
treating?
1.Rheumatoid arthritis
2.Chicken pox
3.Diabetes mellitus
4.Adenosine deaminase deficiency
17.The enzyme employed for amplification of DNA
during PCR is commercially obtained from
1.Trichoderma reesei
2.Bacillus licheniformis
3.Streptococcus pyogenes
4.Thermus aquaticus
18.A regulatory body working under MoEF for the
release of transgenic crops is
1.NBPGR
2.GEAC
3.NSC
4.NIPGR
- Cry1 endotoxins obtained from Bacillus
Thuringiensis are effective against
1.Flies
2.Mosquitoes
3.Boll worms
4.Nematodes
20.Cry II Ab and Cry I Ab produce toxins that
Control
1.Tobacco budworms and
nematodes respectively
2.Corn borer and cotton bollworms
respectively
3.Cotton bollworms and corn borer
respectively
4.Nematodes and tobacco budworms
respectively
- Bacillus thuringiensis forms protein crystals
which contain insecticidal protein. This protein
1.Binds with epithelial cells of midgut
of the insect pest ultimately killing it
2.Does not kill the carrier bacterium
which is itself resistant to this toxin
3.Is activated by acid pH of the
foregut of the insect pest
4.Is coded by several genes including the gene cry
- What is true about Bt toxin?
1.Bt protein exists as active toxin in
the Bacillus
2.The inactive protoxin gets
converted into active form in the
insect gut
3.The concerned Bacillus has
antitoxins
4.The activated toxin enters the
ovaries of the pest to sterilise it and
thus prevent its multiplication
- Which one of the following is a correct
statement?
1.The anticoagulant hirudin is being
produced from transgenic Brassica
napus seeds
2.Somatic hybridization involves
fusion of two complete plant cells
carrying desired genes
3″Bt” in “Bt-cotton” indicates that it
is a genetically modified organism
produced through biotechnology
4.”Flavr Savr” variety of tomato has
enhanced the production of
ethylene which improves its taste
- Bt cotton is not:
- A GM plant
- Insect resistant
- A bacterial gene expressing system
- Resistant to all pesticides
- C-peptide of human insulin is:
- A part of mature insulin molecule
- Responsible for formation of disulphide bridges
- Removed during maturation of pro-insulin to insulin
- Responsible for its biological activity.
- GEAC stands for:
- Genome Engineering Action Committee
- Ground Environment Action Committee
- Genetic Engineering Approval Committee
- Genetic and Environment Approval committee
- Alpha-1 antitrypsin is:
- An antacid
- An enzyme
- Used to treat arthritis
- Used to treat emphysema
- A probe which is a molecule used to locate specific sequences in a mixture
of DNA or RNA molecules could be:
- A single stranded RNA
- A single stranded DNA
- Either RNA or DNA
- Can be ss DNA but not ss RNA
- Choose the correct option regarding Retrovirus:
- An RNA virus that can synthesise DNA during infection
- A DNA virus that can synthesise RNA during infection
- A ssDNA virus
- A dsRNA virus
- The site of production of ADA in the body is:
- Bone marrow
- Lymphocytes
- Blood plasma
- Monocytes
- A protoxin is:
- A primitive toxin
- A denatured toxin
- Toxin produced by protozoa
- Inactive toxin
- Pathophysiology is the:
- Study of physiology of pathogen
- Study of normal physiology of host
- Study of altered physiology of host
- None of the above
- The trigger for activation of toxin of Bacillus thuringiensis is:
- Acidic pH of stomach
- High temperature
- Alkaline pH of gut
- Mechanical action in the insect gut
- Golden rice is:
- A variety of rice grown along the yellow river in China
- Long stored rice having yellow colour tint
- A transgenic rice having gene for – carotene
- Wild variety of rice with yellow coloured grains
- In RNAi, genes are silenced using:
- ss DNA
- ds DNA
- ds RNA
- ss RNA
- The first clinical gene therapy was done for the treatment of:
- AIDS
- Cancer
- Cystic fibrosis
- SCID (Servere Combined Immuno Deficiency resulting form
deficiency of ADA)
- ADA is an enzyme which is deficient in a genetic disorder SCID. What is
the full form of ADA?
- Adenosine deoxy aminase
- Adenosine deaminase
- Aspartate deaminase
- Arginine deaminase
- Silencing of a gene could be achieved through the use of:
- short interfering RNA (RNAi)
- antisense RNA
- by both
- none of the above
- The transgenic plant flavr savr tomato carries an artificial gene for
(A) Delay ripening process
(B) Longer shell life
(C) Added flavours
(D) All of these - Biopatents are ______.
(A) Right to use invention
(B) Right to use biological entities
(C) Right to use products
(D) Right to use process - A set standards used to regulate own or community activity in relation to biological world is
(A) Biopotency
(B) Biopiracy
(C) Biowar
(D) Bioethics - Biopiracy means
(A) Use of biopatents
(B) Thefts of plants and animals
(C) Stealing of bioresources
(D) Exploitation of bioresources without authentic permission - Bioethcs is related to
(A) Preventing biopiracy
(B) Regulation of unethical activities like gene cloning in animals
(C) Preventing theft of living materials
(D) Moral guidance to the problems in biology
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