Biotechnology Principals and processes Test Paper by TEACHING CARE online tuition and coaching classes

Biotechnology Principals and processes Test Paper by TEACHING CARE online tuition and coaching classes. Answers are in Bold option.

  1. Three critical research areas of

biotechnology are:

(i) Providing the best catalyst in the form of improved

organism usually a microbe or pure enzyme.

(ii) Creating optimal conditions through engineering for

a catalyst to act, and

(iii) Downstream processing technologies to purify the

protein/organic compound.

Choose the correct statement

  1. i only
  2. ii only
  3. iii only
  4. i, ii, iii


  1. The three options that can be thought

for increasing food production

(i) agro-chemical based agriculture

(ii) organic agriculture; and

(iii) genetically engineered crop-based agriculture

Which of the above option causes soil pollution and decrease of soil fertility

  1. i only
  2. ii only
  3. iii only
  4. i, ii, iii


  1. Which of the following options regarding green revolution is /are correct


  1. The Green Revolution succeeded in tripling the food supply
  2. The Green Revolution was not enough to feed the growing human population.
  • The Increased yields during Green Revolution have partly been due to the use of improved crop varieties, but mainly due to the use of better management practices and use of agrochemicals (fertilisers and pesticides).


  1. i only
  2. ii only
  3. iii only
  4. i, ii, iii



  1. Genetic modification has:

(i) made crops more tolerant to abiotic stresses (cold, drought, salt, heat).

(ii) reduced reliance on chemical pesticides (pest-resistant crops).

(iii) helped to reduce post harvest losses.

(iv) increased efficiency of mineral usage by plants (this prevents early exhaustion of fertility of


(v) enhanced nutritional value of food, e.g., Vitamin ‘A’ enriched rice.


Which of the above options  is /are correct

  1. i, iv only
  2. i,ii only
  3. ii, iii only
  4. i,ii, iii,iv



5.Choose the options given below that are not false


  1. Bt toxin is produced by a bacterium called Bacillus thuringiensis .
  2. Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide

resistance to insects without the need for insecticides;

  1. Bt toxin is a bio-pesticide.
  1. a  only
  2. b only
  3. a, b only
  4. a, b, c only


  1. Select the incorrect staement


  1. Some strains of Bacillus thuringiensis produce proteins that

kill certain insects such as lepidopterans (tobacco budworm, armyworm),

coleopterans (beetles) and dipterans (flies, mosquitoes).

  1. B. thuringiensis form protein crystals during a particular phase of their growth.
  2. These B. thuringiensis  protein crystals contain a toxic insecticidal protein.
  3. the Bt toxin protein exist as active protoxins



  1. The plant ‘Brassica napus’ has been modified to

produce ______.

1.Vitamin A


3.lysine rich seeds


8.The process of RNA interference has been

used in the development of plants resistant to





9.The transgenic animals are those which have

1.foreign DNA in some of their cells

2.foreign DNA in all of their cells

3.foreign RNA in all of their cells

4.Both (A) and (C)

10.Transgenic plants are developed by

1.Introducing foreign genes

2.Introducing gene mutations

3.Deleting certain chromosomes


4.Stopping spindle formation

  1. Cry genes or Bt genes are obtained from

1.Cotton pest

2.Tobacco plant

3.Bacillus thuringiensis

4.E – Coli

12.Genetic engineering has been successfully

used for producing

1.Transgenic models for studying

new treatments for certain cardiac diseases

2.Transgenic mice for testing safety

of polio vaccine before use in


3.Animals like bulls for farm work as

they have super power

4.Transgenic Cow-Rosie which

produces high fat milk for making



13.Tobacco plants resistant to a nematode have

been developed by the introduction of DNA that

produced (in the host cells)


1.An antifeedant

2.A particular hormone

3.Both sense and anti-sense RNA

4.A toxic protein


14.Some of the characteristics of Bt cotton are

1.Medium yield, long fibre and

resistance to beetle pests

2.Long fibre and resistance to aphids

3.High yield and resistance to


4..High yield and production of toxic

protein crystals which kill dipteran



  1. Stirred-tank bioreactors have been designed for

1.Purification of the product

2.Addition of preservatives to the


3.Availability of oxygen throughout

the process

4.Ensuring anaerobic conditions in

the culture vessel


16.The first clinical gene therapy was given for


1.Rheumatoid arthritis

2.Chicken pox

3.Diabetes mellitus

4.Adenosine deaminase deficiency


17.The enzyme employed for amplification of DNA

during PCR is commercially obtained from

1.Trichoderma reesei

2.Bacillus licheniformis

3.Streptococcus pyogenes

4.Thermus aquaticus


18.A regulatory body working under MoEF for the

release of transgenic crops is






  1. Cry1 endotoxins obtained from Bacillus

Thuringiensis are effective against



3.Boll worms



20.Cry II Ab and Cry I Ab produce toxins that


1.Tobacco budworms and

nematodes respectively

2.Corn borer and cotton bollworms


3.Cotton bollworms and corn borer


4.Nematodes and tobacco budworms



  1. Bacillus thuringiensis forms protein crystals

which contain insecticidal protein. This protein

1.Binds with epithelial cells of midgut

of the insect pest ultimately killing it

2.Does not kill the carrier bacterium

which is itself resistant to this toxin

3.Is activated by acid pH of the

foregut of the insect pest

4.Is coded by several genes including the gene cry


  1. What is true about Bt toxin?

1.Bt protein exists as active toxin in

the Bacillus

2.The inactive protoxin gets

converted into active form in the

insect gut

3.The concerned Bacillus has


4.The activated toxin enters the

ovaries of the pest to sterilise it and

thus prevent its multiplication


  1. Which one of the following is a correct


1.The anticoagulant hirudin is being

produced from transgenic Brassica

napus seeds

2.Somatic hybridization involves

fusion of two complete plant cells

carrying desired genes

3″Bt” in “Bt-cotton” indicates that it

is a genetically modified organism

produced through biotechnology

4.”Flavr Savr” variety of tomato has

enhanced the production of

ethylene which improves its taste



  1. Bt cotton is not:
  2. A GM plant
  3. Insect resistant
  4. A bacterial gene expressing system
  5. Resistant to all pesticides


  1. C-peptide of human insulin is:
  2. A part of mature insulin molecule
  3. Responsible for formation of disulphide bridges
  4. Removed during maturation of pro-insulin to insulin
  5. Responsible for its biological activity.


  1. GEAC stands for:
  2. Genome Engineering Action Committee
  3. Ground Environment Action Committee
  4. Genetic Engineering Approval Committee
  5. Genetic and Environment Approval committee


  1. Alpha-1 antitrypsin is:
  2. An antacid
  3. An enzyme
  4. Used to treat arthritis
  5. Used to treat emphysema
  6. A probe which is a molecule used to locate specific sequences in a mixture

of DNA or RNA molecules could be:

  1. A single stranded RNA
  2. A single stranded DNA
  3. Either RNA or DNA
  4. Can be ss DNA but not ss RNA


  1. Choose the correct option regarding Retrovirus:
  2. An RNA virus that can synthesise DNA during infection
  3. A DNA virus that can synthesise RNA during infection
  4. A ssDNA virus
  5. A dsRNA virus


  1. The site of production of ADA in the body is:
  2. Bone marrow
  3. Lymphocytes
  4. Blood plasma
  5. Monocytes


  1. A protoxin is:
  2. A primitive toxin
  3. A denatured toxin
  4. Toxin produced by protozoa
  5. Inactive toxin


  1. Pathophysiology is the:
  2. Study of physiology of pathogen
  3. Study of normal physiology of host
  4. Study of altered physiology of host
  5. None of the above


  1. The trigger for activation of toxin of Bacillus thuringiensis is:
  2. Acidic pH of stomach
  3. High temperature
  4. Alkaline pH of gut
  5. Mechanical action in the insect gut
  6. Golden rice is:
  7. A variety of rice grown along the yellow river in China
  8. Long stored rice having yellow colour tint
  9. A transgenic rice having gene for – carotene
  10. Wild variety of rice with yellow coloured grains


  1. In RNAi, genes are silenced using:
  2. ss DNA
  3. ds DNA
  4. ds RNA
  5. ss RNA


  1. The first clinical gene therapy was done for the treatment of:
  2. AIDS
  3. Cancer
  4. Cystic fibrosis
  5. SCID (Servere Combined Immuno Deficiency resulting form

deficiency of ADA)


  1. ADA is an enzyme which is deficient in a genetic disorder SCID. What is

the full form of ADA?

  1. Adenosine deoxy aminase
  2. Adenosine deaminase
  3. Aspartate deaminase
  4. Arginine deaminase


  1. Silencing of a gene could be achieved through the use of:
  2. short interfering RNA (RNAi)
  3. antisense RNA
  4. by both
  5. none of the above
  6. The transgenic plant flavr savr tomato carries an artificial gene for
    (A) Delay ripening process
    (B) Longer shell life
    (C) Added flavours
    (D) All of these
  7. Biopatents are ______.
    (A) Right to use invention
    (B) Right to use biological entities
    (C) Right to use products
    (D) Right to use process
  8. A set standards used to regulate own or community activity in relation to biological world is
    (A) Biopotency
    (B) Biopiracy
    (C) Biowar
    (D) Bioethics
  9. Biopiracy means
    (A) Use of biopatents
    (B) Thefts of plants and animals
    (C) Stealing of bioresources
    (D) Exploitation of bioresources without authentic permission
  10. Bioethcs is related to
    (A) Preventing biopiracy
    (B) Regulation of unethical activities like gene cloning in animals
    (C) Preventing theft of living materials
    (D) Moral guidance to the problems in biology